Campylobacter jejuni (c.jejuni) Campylobacter jejuni resistance is not strong, easily dry, direct sunlight and disinfectants which kill the weak, 56 ℃ 5 minutes to be killed. Erythromycin, neomycin, gentamicin, tetracycline, chloramphenicol, kanamycin and other antibiotics sensitive. Found a lot of resistant strains in recent years. Campylobacter jejuni as antigen structure and intestinal bacteria with o, h and k antigens. Under the o antigen of Campylobacter jejuni can be put into more than 45 serotypes, No. 11,12 and 18 most common serotypes. Campylobacter jejuni is a variety of animals such as cattle, sheep, dogs and birds of the normal dwelling bacteria. In their reproductive tract or a large number of intestinal bacteria, it can be by birth or fecal contamination of food and water. Generally susceptible from the group, children under 5 years of age the highest incidence in summer and autumn common. Flies also play an important role of the media. Also by the contact. Maternal infection can be transmitted to the fetus during delivery. Isolated Taking antibiotics before or cervical mucus and other diarrheal stool, 3 hours was inoculated with a highly _select_ive plate culture (in Brucella agar-based isolation plus 10% sheep blood, while adding vancomycin per liter 10mg, polymyxin b2500iu, amphoterin 13mg, cephalocin 15mg), and then put inside the aquarium (containing 85% n2, 10% co2, 5% o2), hatched box _set_ 42 ℃ for 48 hours, the _select_ion of suspicious colonies then biochemical reactions and serum agglutination test to make the final identification. Serology Week after on_set_, serum antibodies can occur, mainly lgm, available indirect hemagglutination test and indirect immunofluorescence assay and other detection of specific antibody titers, the titers of normal or carriers of up to 1:2 to 1:8, acute patients antibody titers of up to 1:8 ~ 1:32, recovery of up to 1:80 ~ 1:320 or more. Since the serum antibody titer is not high, double serum test should be taken to 4 times higher potency as a diagnosis.
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No. 2
Campylobacter jejuni (c.jejuni) Campylobacter jejuni resistance is not strong, easily dry, direct sunlight and disinfectants which kill the weak, 56 ℃ 5 minutes to be killed. Erythromycin, neomycin, gentamicin, tetracycline, chloramphenicol, kanamycin and other antibiotics sensitive. Found a lot of resistant strains in recent years, and multiple drug resistance strains. Campylobacter jejuni as antigen structure and intestinal bacteria with o, h and k antigens. Under the o antigen of Campylobacter jejuni can be put into more than 45 serotypes, No. 11,12 and 18 most common serotypes. Campylobacter jejuni is a variety of animals such as cattle, sheep, dogs and birds of the normal dwelling bacteria. In their reproductive tract or a large number of intestinal bacteria, it can be by birth or fecal contamination of food and water. Generally susceptible population, children under 5 years of age the highest incidence in summer and autumn common. Flies also play an important role of the media. Also by the contact. Maternal infection can be transmitted to the fetus during delivery. Campylobacter is a zoonotic pathogen Yan Yan, can cause a variety of human and animal diseases occur, and is a food borne pathogen Yin is considered the cause of human bacterial diarrhea worldwide mainly on Campylobacter Yin pathogenic mechanism of more and more. The causative factors include adhesion, invasion, produce toxins, and mechanisms of the four aspects of molecular simulation, molecular simulation by molecular mechanisms can cause the most serious complications of a Guillain Barre syndrome. Campylobacter Yin tension by enterotoxin producing cells, cell lethal toxin and cell expansion of toxins and disease Isolated Taking antibiotics before or cervical mucus and other diarrheal stool, a short sample with a cotton swab dampened with the inclusion of the sample, such as vancomycin release 10mg, polymyxin b2500iu, amphoterin 13mg, cephalocin 15mg in the enrichment broth culture, inoculated at Columbia blood (5% off the fiber sheep blood) plates, culture 24H observation, if you _select_ a single Junzhu bacteria and purified before use hippuric acid and oxidase testing, can afford to do the serum agglutination test (cost high). screening will be positive, and PCR testing of suspected isolates (16S, mapA, hipO) eventually identified as Campylobacter jejuni. Culture Collection 20 degrees in the negative can generally be stored for 6 months, negative 70 degrees save time can be freeze-dried for up to two years. Serology Week after on_set_, serum antibodies can occur, mainly lgM, available indirect hemagglutination test and indirect immunofluorescence assay and other detection of specific antibody titers, the titers of normal or carriers of up to 1:2 to 1:8, acute patients antibody titers of up to 1:8 ~ 1:32, recovery of up to 1:80 ~ 1:320 or more. Since the serum antibody titer is not high, double serum test should be taken to 4 times higher potency as a diagnosis.
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Wikipedia Daquan
Campylobacter jejuni campylobacter jejuni Gram-negative bacteria. Can not ferment sugars, micro-aerobic system, spiral or S-shaped tapering at both ends. Pathogenic enteritis in children. Violations of the bacteria but also easy to lower the resistance of older infants and children, can occur even systemic infection or meningitis. Can be isolated from the stool of patients with bacteria, can also be diagnosed based on elevated serum antibody titers.