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germ
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高氏1号培养基
可溶性淀粉 2g
kno3 0.1g
k2hpo4 0.05g
mgso47h2o 0.05g
nacl 0.05g
feso47h2o 0.001g (母液)
琼脂 2g
自来水 100ml
ph 7.2~7.4
灭菌 1.05kg/cm2,20min
配制时,先用少量冷水,将淀粉调成糊状,倒入少于所需水量的沸水中,在火上加热,边搅拌边依次逐一溶化其他成分,溶化后,补足水分到100ml,调ph。
高温灭菌后,倒平皿前加入10%酚2滴至100ml培养基中混匀,将培养基倒入平皿内约15ml/皿。