Rapid hydrogen sulfide (h2s) test agar Method a liquid: Brucella broth 970ml Anhydrous disodium hydrogen phosphate 1.18g Anhydrous potassium dihydrogen phosphate 0.23g Agar 2g Heated to dissolve, autoclave, 121 ℃ 15min. B 10% solution by adding ferrous sulfate (including 7h2o), c 10% solution of sodium metabisulfite, d 10% liquid sodium pyruvate. The b, c, d solutions are freshly prepared, using membrane filtration sterilization. Sterilization solution of this mixture and then into a 1ml solution, sterile transfer ph to 7.3, sterile packaging and each tube 3ml, plugged back.
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No. 2
Rapid hydrogen sulfide (H2S) test agar Method A solution: Brucella broth 970mL Anhydrous disodium hydrogen phosphate 1.18g Anhydrous potassium dihydrogen phosphate 0.23g Agar 2g Heated to dissolve, autoclave, 121 ℃ 15min. Adding 10% ferrous sulfate solution B (containing 7H2O), C 10% solution of sodium metabisulfite, D liquid 10% sodium pyruvate. The B, C, D solutions are freshly prepared, using membrane filtration sterilization. 1mL sterilization solution of this mixture and then into the A solution, pH adjusted to 7.3, sterile, aseptic packaging and each tube 3mL, plugged back.